GE Cytiva AKTA Pure 25M FPLC Chromatography System + Frac/PC 2017
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Description
SEPARATION
GE Cytiva AKTA pure 25 M Chromatography System | Protein Purification
Modular benchtop FPLC chromatography system for fast purification of proteins, peptides, and nucleic acids at laboratory scale.
QC PASS
Tested & Working
At a glance
Manufacturer
GE Healthcare / Cytiva
Model
ÄKTA pure 25 M
Fraction Collector F9-C
Part / Catalog #
29018226
29015408
Serial #
2208444
2200996
Year / DOM
2017
Condition
Used - Excellent
What's Included
GE Cytiva ÄKTA pure 25 M chromatography system
Fraction Collector F9-C
Dell desktop computer with Unicorn 7.6 software
Power cords, Ethernet cable
UniNet-9 control cable
Nothing else is included unless shown in the photos
Condition & Testing
Unit powers on and UNICORN software loads to a Ready state with both system pumps, all valves, and the U9-M UV monitor recognized; no cracks or damage; flow-path calibration and performance qualification are the responsibility of the buyer.
Specifications
Specs from manufacturer literature for reference.
Specifications
| Manufacturer | GE Healthcare / Cytiva |
| Model | F9-C flexible fraction collector |
| Condition | Used |
| Serial Number | 2200996 |
| Mpn | 29018226 , 29014834 , 29027743 |
| System type | Laboratory-scale FPLC / liquid chromatography purification system |
| Configured modules | V9-Inj, V9-IAB, V9-M, V9-L, V9-C, V9-pH, V9-O, U9-M, C9, L9, F9-C, P9 A, P9 B, R9, M9 |
| System pumps | Two high-performance pumps (P9 A and P9 B), one pair of pump heads each |
| Flow rate range | 0.001–25 mL/min (normal); up to 50 mL/min for column packing |
| Max operating pressure | 200 bar (2900 psi, 20 MPa) |
| Uv monitor | U9-M multi-wavelength; 190–700 nm, up to 3 wavelengths simultaneously |
| Uv flow cell path lengths | 0.5, 2 (included at delivery), and 10 mm |
| Uv lamp | High-intensity xenon lamp; typical operating time ~5000 h; auto-calibrated at power-on |
| Conductivity monitor | C9 (in-line, for gradient verification) |
| System pressure monitor | R9 (continuous; auto-adjusts flow to avoid pressure limits) |
| Mixer | M9; 1.4 mL chamber included (optional 0.6 and 5 mL); in-line filter mounted inside |
| Injection valve | V9-Inj; supports sample loops, Superloops, and direct-to-column application |
| Sample loop | 500 µL delivered with system |
| Inlet valve | V9-IAB (selection between two A and two B positions) |
| Column valve | V9-C (built-in bypass and reverse-flow functionality) |
| Ph valve | V9-pH (in-line pH monitoring, calibration, and storage) |
| Outlet valve | V9-O |
| External air sensor | L9 (column protection) |
| Chromatography techniques | Affinity, size exclusion (SEC), ion exchange, hydrophobic interaction, and reversed phase (RPC) |
| Wetted materials | Biocompatible; resistant to commonly used solvents |
| Power input | 100–240 VAC, 50/60 Hz |
| Power consumption | 1100 VA (max) |
| Mains fuse | T10AL250V |
| Mains out | As mains in (2 A max) |
| Communication | UniNet-9 instrument interface; Ethernet (IP 10.1.1.1) |
| Ingress protection | IP21 |
| Weight | 48 kg basic configuration; 53 kg fully equipped |
| Code no. | 29015408 |
| Year / dom | 2017/02 |
| Collection format | Deep-well plates and/or tubes |
| Outlet positions | 10 outlet positions + waste |
| Voltage | 32 V via UniNet-9 interface |
| Power | 18 VA max |